• 作者： 高銘欽; 武家安; 白壽雄
• 作者服務機構： 國防醫學院生化系
• 中文摘要： 以噬菌體入EMBL4為載體，經由活體外裝填(In vitro Packaging)技術，核酸雜化及分子選殖操作，對應於人體磷酸果
  糖激?氮端部份的基因已被分離純化出來。核酸序列分析及比較的結果顯示人體與免肌磷酸果糖激?基因的第一個表現
  序列大小完全相同，此二者在核酸序列的層次上有11個氮鹼基產生變異，但其中有8個屬於靜態突變(Silent Mutation)；而
  在胺酸序列的層次上只有第9與13兩個位置的胺酸不同，但也都屬於保留性置換(Conservative Replacement)，分別為精胺
  酸→離胺酸及纈胺酸→異白胺酸。有趣的是在這些變異當中氮鹼基的使用在人體磷酸果糖激?基因皆為A或T，而免肌
  磷酸果糖激?基因在同一位置上則為C或G。這種特殊保留性的現象也發生在此二基因表現序列的兩側側翼序列中。經
  由單位演化時期（UEP）的計算，本研究提供為探討哺乳動物基因演化的良好模型。n
• 英文摘要： A phosphofructokinase gene was screened and cloned from a human genomic library prepared in thelambda EMBL4 phage vector. DNA sequencing shows that the first exon of this human phosphofructo-kinase gene is identical in length and highly homologous in sequence to that of a rabbit phosphofructo-kinase gene. Two amino acid replacements are indicated, an Arg－－＞Lys and a Val－－＞Ile at positions9 and 13, respectively. Eleven base substitutions, 8 of them silent, are identified. Surprisingly, at ten ofthese sites, complete bias for A's and T's in the human gene and C's and G's in the rabbit gene are seen.Strong conservation is also observed in the 5’untranslated region and for the first 15 base pairs in theintron. All the nine variant nucleotides in these regions are, again, A's and T's in the human gene and G'sand C's in the rabbit gene. The unit evolutionary period of change between the first exon of rabbit andhuman phosphofructokinase genes is estimated as 2.3 million years at silent sites and 15.6 million yearsat replacement sites.
• 中文關鍵字： phosphofructokinase gene; human; exon motif; gene evolution
• 英文關鍵字： --