- 作者: Yihang Wu; Fang Wang; Qunxiong Zheng; Longxi Lu; Hongtian Yao; Changxin Zhou; Xiumei Wu; Yu Zhao
- 作者服務機構: 1 Department of Traditional Chinese Medicine and Natural Drug Research, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310031, China; ; 2 Hangzhou Huqingyutang Pharmaceutical Co., Ltd.. Hangzhou 310002, China; ; 3 College of Food Science, Biotechnology & Environmental Engineering, Zhejiang Gongshang University, Hangzhou 310035, China; ; 4 Zhejiang Provincial Center for Disease Prevention and Control, Hangzhou 310009, China; ; 5 Pathology Department, First Affiliated Hospital, ZheJiang University College of Medicine, Hangzhou 310003, China
- 中文摘要: --
- 英文摘要: The hepatoprotective activities of total flavonoids of Laggera alata (TFLA) were evaluated by carbon tetrachloride (CCl4)-induced injury in primary cultured neonatal rat hepatocytes and in rats with hepatic damage. In vitro, TFLA at a concentration range of 1-100 μg/ml improved cell viability and inhibited cellular leakage of two enzymes, hepatocyte aspartate aminotransferase (AST) and alanine aminotransferase (ALT), caused by CCl4. In vivo, oral treatment with TFLA at doses of 50, 100, and 200 mg/kg significantly reduced the levels of AST, ALT, total protein, and albumin in serum and the hydroxyproline and sialic acid levels in liver. Histopathological examinations revealed that liver damage were improved when treated with TFLA. Meanwhile, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide radicals scavenging activities of TFLA were also determinated. To understand the exact components of TFLA responsible for the hepatoprotective effect, nine flavonoid compounds were isolated and identified from TFLA. In conclusion, the present investigation was the first to verify the hepatoprotective effect of L. alata in vitro and in vivo. The hepatoprotective action of TFLA is likely related to its potent antioxidative and anti-inflammatory activity. Neutralizing reactive oxygen species by nonenzymatic mechanisms and enhancing the activity of original natural hepatic-antioxidant enzymes may be the main mechanisms of TFLA against CCl4-induced injury.
- 中文關鍵字: --
- 英文關鍵字: antioxidative activity, carbon tetrachloride, flavonoids, hepatoprotective activity, Laggera alata