- 作者: 柯南靖
- 作者服務機構: 國立中興大學植物病理系
- 中文摘要: LR White 包埋劑首次成功地應用在植物病毒之研究,本研究是以胡瓜嵌紋病毒及豆科黃化嵌紋病毒感染之蝴蝶蘭與豌豆組織為材料,發現以 protein A-gold 來標示經此包埋劑包埋之組織,其效果良好,膠金顆粒可以非常專一地附著於胡瓜嵌紋病毒之顆粒及豆科黃化嵌紋病毒引起之內含體上。本法之優點在於 LR White 包埋劑易於使用以及其可廣泛地應用於植物病毒之細胞學研究。
- 英文摘要: Currently available immunostaining methods for plant virus infections are time consuming and insome cases extra facilities are needed for embedding and staining. Some methods even cause more non-specific labelling. LR White is introduced as an embedding medium for immunolabelling of plant virusinfections. Protein A-gold (15 nm in diameter) is used as a label for cucumer mosaic virions and beanyellow mosaic virus-induced cylindrical inclusions. The advantages of this embedding medium are that itis very easy to use and can be widely applied to plant virus infections in cytological laboratories.
- 中文關鍵字: LR White; protein A-gold
- 英文關鍵字: --