- 作者: Jen Her Lu; Jenn Kan Lu; Sie Lin Choo; Yu-Chi Li; Huei-Wen Yeh; Jau-Fen Shiue; Vincent C. Yeh
- 作者服務機構: Department of Pediatrics & Pediatric Cardiology, Veterans General Hospital-Taipei, National Yang Ming University, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要:
Zebrafish tbx5 expresses in the heart, pectoral fins and eyes of zebrafish during embryonic development. In zebrafish, injection of tbx5 morpholino antisense RNA caused changes of heart conformation, defect of heart looping, pericardium effusion, dropsy of ventral position
and decreased heart rate. We suggested that cardiac myogenesis genes might be responsible for this phenomenon. Morpholino antisense RNA which against the initiation site of tbx5 gene was designed in order to knockdown the expression of tbx5, and the results were analyzed by wholemount in situ hybridization and quantitative real-time PCR. Expression of cardiac myogenesis genes amhc, vmhc and cmlc2 were expressed constantly at the early embryonic development and reached its highest rate right before cardiac looping initiated. These cardiac myogenesis genes showed insufficient expressions within different heart defect embryos. Moreover, vmhc showed ectopic expression
in addition to heart looping defect in heart defective embryos at 36 hpf. Our data suggests that the heart failure caused by the knockdown of tbx5 gene might result from the down-regulation of cardiac myogenesis genes. - 中文關鍵字: --
- 英文關鍵字: tbx5; Cardiac myogenesis genes; Cardiac looping