- 作者: Pei-Chang Sun; Ching Tzao; Ban-Hen Chen; Chen-Wei Liu; Cheng-Ping Yu; Jong-Shiaw Jin
- 作者服務機構: Graduate Institute of Pathology and Parasitology, Tri-Service General Hospital, National Defense Medical Center, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
Background: Histone deacetylases and histone acetyl transferases covalently modify histone
proteins, consequentially altering chromatin architecture and gene expression.
Methods: The effects of suberoylanilide hydroxamic acid, a HDAC inhibitor, on 320 HSR colon
cells were assessed in 320 HSR colon cancer cells.
Results: Concentration and time-dependent inhibition of 320 HSR cell proliferation was
observed. Treatment of 320 HSR cells with 5 μM SAHA for 72 h significantly inhibited their
growth by 50% as compared to that of the control. Fluorescence-activated cell sorting analysis
demonstrated significant inhibition of cell cycle progression (sub-G1 arrest) and induction of
apoptosis upon various SAHA concentrations after 48 h. In addition, the anti-apoptosis proteins,
survivin and Bcl-xL, were significantly inhibited by SAHA after 72 h of treatment.
Immunocytochemistry analysis revealed that SAHA-resistant cells were positive for cyclin A
(85%), ki-67 (100%), p53 (100%), survivin (100%), and p21 (90%) expression. Furthermore, a
significant increase cyclin A-, Ki-67-, p53-, survivin-, and p21-positive cells were noted in
SAHA-resistant tumor cells.
Conclusion: Our results demonstrated for the first time in 320 HSR colon adenocarcinoma cells
that SAHA might be considered as an adjuvant therapy for colon adenocarcinoma. - 中文關鍵字: --
- 英文關鍵字: --