- 作者: 吳芳禎 ; 林家立
- 作者服務機構: 中興大學化學系
- 中文摘要: The phospholipase A2-catalyzed hydrolysis of phosphatidylcholine in organic solvents is described. The effects of various sources of enzymes are discussed; among five phospholipases A2, bee venom enzyme has the greatest activity, Naja naja venom and Naja mocambique enzymes have moderate activities, and pancreatic enzymes have the least activities. The effects of adducts such as alcohols, ether, and bovine serum albumin in chloroform on this catalysis are discussed; adducts, such as ether or alcohol, in small proportions increased the hydrolysis rate, but in large proportions inhibited hydrolysis. Bovine serum albumin increased slightly the phospholipase A2 catalysis rate. From the effect of temperature on this catalysis in chloroform, the substrate conformation at the .alpha.- methylene region of acyl chains is a major factor for activation of phospholipases A2, confirming our previous conclusion that the substrate conformation is an important factor in the activation of phospholipase A2.
- 英文摘要: --
- 中文關鍵字: Phospholipase; Organic Solvent; Activation; Hydrolysis; Enzyme Activity; Phosphatidylcholine; Lecithin
- 英文關鍵字: 磷脂;有機溶劑;活化;水解;酵素活性;卵磷脂