- 作者: Chun-yi Chiang and Chihchen Chen
- 作者服務機構: 1. Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan 2. Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan
- 中文摘要:
- 英文摘要:
Extracellular vesicles (EVs) are cell-derived membrane-bound vesicles that serve a means of cell-cell communication. Studying EVs at a single-particle level is important because EVs are inherently heterogeneous. Novel micro- and nanotechnological tools have open opportunities for realizing single-EV measurements exploiting their biochemical, electrical, mechanical, and/or optical properties. This review summarizes the recent development of technologies toward sorting and analyzing single EVs. Sorting EVs into a more homogeneous subset relaxes the sensitivity and throughput required on the EV detection, and hence related techniques are also included in this review. These exciting technologies are on the rise and will expand our understanding of EVs and their applications in the near future. - 中文關鍵字:
- 英文關鍵字: Extracellular vesicle, Single particle, Exosome, Microvesicle, Electrophoresis, Raman spectroscopy, Atomic force microscopy, Digital PCR