- 作者: A Luquita, L Urli; M J Svetaz; A M Gennaro; M E Giorgetti; G Pistone; R Volpintesta; S Palatnik; M Rasia
- 作者服務機構: Catedra de Fisica Biologica, Facultad de Ciencias Medicas, Universidad Nacional de Rosario, Santa Fe 3100, 2000 Rosario, Argentina
- 中文摘要: --
- 英文摘要:
Background. Hyaluronic acid (HA) is present in many tissues; its presence in serum may
be related to certain inflammatory conditions, tissue damage, sepsis, liver malfunction
and some malignancies. In the present work, our goal was to investigate the significance
of hyaluronic acid effect on erythrocyte flow properties. Therefore we performed in vitro
experiments incubating red blood cells (RBCs) with several HA concentrations.
Afterwards, in order to corroborate the pathophysiological significance of the results
obtained, we replicated the in vitro experiment with ex vivo RBCs from diagnosed
rheumatoid arthritis (RA) patients, a serum HA-increasing pathology.
Methods. Erythrocyte deformability (by filtration through nucleopore membranes) and
erythrocyte aggregability (EA) were tested on blood from healthy donors additioned with
purified HA. EA was measured by transmitted light and analyzed with a mathematical
model yielding two parameters, the aggregation rate and the size of the aggregates.
Conformational changes of cytoskeleton proteins were estimated by electron
paramagnetic resonance spectroscopy (EPR).
Results. In vitro, erythrocytes treated with HA showed increased rigidity index (RI) and
reduced aggregability, situation strongly related to the rigidization of the membrane
cytoskeleton triggered by HA, as shown by EPR results. Also, a significant correlation
(r: 0.77, p<0.00001) was found between RI and serum HA in RA patients.
Conclusions. Our results lead us to postulate the hypothesis that HA interacts with the
erythrocyte surface leading to modifications in erythrocyte rheological and flow
properties, both ex vivo and in vitro. - 中文關鍵字: --
- 英文關鍵字: --