- 作者: 羅禮強,朱啟元,林芳民,潘彥如,李耀坤,林敬哲
- 作者服務機構: Department of Chemistry, National Taiwan University, Taipei 106, Taiwan, R.O.C.
- 中文摘要: Due to their industrial interest and significance, developing a rapid method for screening b-xylosidase activities would certainly facilitate the research and improve production in xylan-related industries especially in the pulp and paper industries. Here we report the synthesis of two activity probes, LCL-6X and -12X, for b-xylosidase. They both carry a b-xylopyranosyl recognition head, which is linked to a latent trapping device consisting of a 2-fluoromethylphenoxyl group and a biotin reporter group. They differ only in the length of their hydrophobic linker. A model study using a b-xylosidase from T. koningii demonstrates that both probes could successfully label the target hydrolase and give rise to biotinylated proteins. They could thus potentially become a powerful tool in screening b-xylosidase from microbial sources.
- 英文摘要: --
- 中文關鍵字: Quinone methide; Xylosidases; Activity probe; Xylan; Labeling; Proteome; Biotin; Mechanism-based.
- 英文關鍵字: --