- 作者: 王西華
- 作者服務機構: 國立臺灣大學
- 中文摘要: With the technique of nutritionally forced mating, two strains, 54-3 and 54-7,which had been distributed in Taiwan for mushroom cultivation, were used for thedetermination of mating types. The result would be available for the breeding ofthe mushroom, Agaricus bisporus. 1. Nutritionally forced mating requires auxotrophic mutants. Therefore, themutagen, nitrosoguanidine (NTG), was used for mutagenesis. When the lethal rateswere in the range of 90-99%, fertile heterokaryotic strains showed the mutationfrequencies at the magnitude of order of 0.1%, while sterile homokaryotic strains,which were supplied by J. R. Raper, indicated 0.01% or less. These mutation frequ-encies are far from those in the mutagenesis by X ray, which were presented inthe previous paper. 2. With the use of fertile heterokaryotic strains, 54-3, 54-7 and M , and alsosterile homokaryotic ones, P , and P , the writer obtained nine auxotrophic mutants,one adenine-less (547-C-8), two proline-less (547-C-22, 547-C-2), two histidine-less(547-C-66, 547-C-92), two unknown requirement (543-19-3a, P -102-85) and fourundetermined. 3. It appears from the result of forced mating with these mutants that (a) twomating types of the strain 54-7 was separated, (b) there is, at least, one uncommonmating type between the strains, 54-3 and 54-7 and (c) at least one mating type ofthe strain, either 54-3 or 54-7, is different from that of homokaryotic P . 4. In short, when one defines that the mating types of 54-3, 54-7 and P are A A , A AY and A , respectively, there, may be A A A A , but the relationamong A , A and A is unknown.
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