- 作者: 趙德讓
- 作者服務機構: 國防醫學院三軍總醫院病理部
- 中文摘要:
勞斯氏肉瘤病毒之Prague B病毒株的基因序列已被測知,而利用oligo(dT)基始(Primer)和反轉謄錄法(Reverse
transcription method),也已建立此種病毒株感染細胞之Poly A+ RNA的cDNA銀行,並篩檢出含有病毒長段的終端
重覆序列(LTR)cDNA株,經由研究數株cDNA之基因序列,發現有兩株在LTR的R及U3區,有特殊的突變。比
較分析各株cDNA和原始病毒LTR基因序列之異同,以探討此突變之發生原因,結果證實反轉謄錄?(Reverse tran-
scriptase)具有錯誤傾向的假說。 - 英文摘要: The cDNA library of the polyA+ RNA from Prague B strain of RSV infected cells was constructedby the use of oligo(dT) primer and the reverse transcription method. The clones containing the LTRsequences were studied. The DNA sequences of several clones were studied and compared with the cDNAsequence of the long terminal repeat (LTR) region of a PrB strain of Rous sarcoma virus (RSV) which hadpreviously been determined. By the analyses of several different cDNA clones, mutations were detectedin the R region as well as in the U3 region of the LTR in two different cDNA clones. By comparing theDNA sequences of these cDNA clones with the original viral LTR, the error-prone hypothesis of the RSVreverse transcriptase was confirmed.
- 中文關鍵字: cDNA sequence; Rous sarcoma virus
- 英文關鍵字: --