- 作者: Hailan Li; Jong-Hyuk Lee; Su Yeon Kim; Hye-Young Yun; Kwang Jin Baek; Nyoun Soo Kwon; Yoosik Yoon; Ji Hoon Jeong; Dong-Seok Kim
- 作者服務機構: Departments of Biochemistry, Chung-Ang University College of Medicine, Seoul, Republic of Korea
- 中文摘要: --
- 英文摘要:
Background: Phosphatidylcholine (PPC) formulation is used for lipolytic injection, even
though its mechanism of action is not well understood.
Methods: The viability of 3T3-L1 pre-adipocytes and differentiated 3T3-L1 cells was
measured after treatment of PPC alone, its vehicle sodium deoxycholate (SD), and a PPC
formulation. Western blot analysis was performed to examine PPC-induced signaling
pathways.
Results: PPC, SD, and PPC formulation significantly decreased 3T3-L1 cell viability in a
concentration-dependent manner. PPC alone was not cytotoxic to CCD-25Sk human
fibroblasts at concentrations <1 mg/ml, whereas SD and PPC formulation were cytotoxic.
Western blot analysis demonstrated that PPC alone led to the phosphorylation of the stress
signaling proteins, such as p38 mitogen-activated protein kinase and c-Jun N-terminal kinase,
and activated caspase-9, -8, -3 as well as cleavage of poly(ADP-ribose) polymerase. However,
SD did not activate the apoptotic pathways. Instead, SD and PPC formulation induced cell
membrane lysis, which may lead to necrosis of cells.
Conclusions: PPC results in apoptosis of 3T3-L1 cells. - 中文關鍵字: --
- 英文關鍵字: adipocytes, apoptosis, caspases, mesotherapy, PPC