- 作者: Chien-Jui Cheng; Yu-Chih Wu; Jye-An Shu; Thai-Yen Ling; Hung-Chih Kuo; Jui-Yu Wu; Ε. E. Chang; Shyh-Chern Chang; Yen-Hua Huang
- 作者服務機構: 1 Department of Pathology, Taipei Medical University, Taipei, Taiwan; ; 2 Department of Biochemistry, Taipei Medical University, Taipei, Taiwan; ; 3 Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan; ; 4 Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan; ; 5 Institute of Pharmacology, College of Medicine, National Taiwan University, Taipei, Taiwan; ; 6 Institute of Cellular and Organismic Biology, Academia S璯ica, Taipei, Taiwan; ; 7 Department of Pathology and Laboratory Medicine, Taoyuan Veterans Hospital, Taoyuan, Taiwan
- 中文摘要: --
- 英文摘要: Testicular germ cell tumors (TGCTs), comprised of seminomas and non-seminomas, are derived from premalignant and noninvasive intracellular germ cell neoplasias. Among TGCTs, seminomas are believed to resemble a transformed state of primordial germ cells (PGCs) and are known to exhibit a gene expression profile similar to that of embryonic stem (ES) cells, such as transcription factor OCT-4. OCT-4 has recently been recognized as a diagnostic marker for clinical aspects of seminomas. However, the role of the OCT-4 protein in seminomas has not been clarified. To determine a possible role of the OCT-4 protein in seminomas, in this paper, we studied a series of 41 testicular tumor tissues and four cell lines by immunohis-tochemistry, Western blotting, and reverse-transcriptase polymerase chain reaction (RT-PCR) to examine the expression and distribution of the OCT-4 transcription factor in seminomas. By utilizing immuno-histochemical staining and Western blotting, we demonstrated that the OCT-4 transcription factor was aberrantly localized in the cytoplasm and nuclei of cells in the collected seminoma tissues. This observation was further confirmed using immunocytochemical staining of NCCIT (seminoma-embryonal carcinoma) and NT2 (embryonal carcinoma) cells. In addition, the RT-PCR results indicated that Oct-4 mRNA was relatively highly expressed in NCCIT, NT2 cells, and seminoma tissues when compared with human embryonic stem cells. The aberrant expression and distribution of the OCT-4 transcription factor in seminomas may provide some important clues concerning the cell transformation between germ line stem cells (like PGC) and testicular germ cell tumors.
- 中文關鍵字: --
- 英文關鍵字: cytosolic distribution, OCT-4 transcription factor, seminoma, testicular germ cell tumors