- 作者: 謝哲宗; 何志?; 彭瑞貞; 陳豪勇; 雍建輝
- 作者服務機構: 國立陽明醫學院微生物及免疫研究所; 榮民總醫院醫學研究部; 衛生署預防醫學研究部; 榮民總醫院血庫
- 中文摘要: 在測定不同組合之PHA、OK432及TPA對人體丙種干擾素之誘導效率時,發現如果三種同時使用比PHA (10μg/ml)及TPA (5μg/ml)或OK432 (10μg/ml)單獨所獲之產量增加2~3倍,用此法生產之干擾素,能為pH 2.0所破壞,具有種之專一性,為T細胞所生產及其作用可被丙種干擾素抗體所中和。
- 英文摘要: Phytohemagglutinin (PHA), picibanil (OK432) and tumor promoting agent (TPA) were tested invarious combinations for optimal induction of human interferon-γ (HuIFN-γ). It was found that the useof a mixture of all 3 inducers resulted in IFN production 2-3 times higher than either PHA(10μg/ml) incombination with TPA (5μg/ml) or picibanil(10μg/ml) alone. The IFN was produced by T lymphocytesand could be neutralized by specific IFN-y antiserum. It was pH 2.0 labile and species specific.
- 中文關鍵字: --
- 英文關鍵字: --