• 作者： Nádia Calvo Martins Okuyama, Fernando Cezar-dos-Santos, Érica Romão Pereira, Kleber Paiva Trugilo, Guilherme Cesar Martelossi Cebinelli, Michelle Mota Sena, Ana Paula Lombardi Pereira, Adriano Martin Felis Aranome, Luis Fernando Lasaro Mangieri, Rodolfo Sanches Ferreira, Maria Angelica Ehara Watanabe and Karen Brajão de Oliveira
• 作者服務機構： Laboratory of molecular genetics and immunology, Department of Pathological Science, Londrina State University, Londrina, PR, Brazil
• 中文摘要：
• 英文摘要：
  Background
  Human papillomavirus (HPV) is the most common sexually transmitted virus in women worldwide. The persistence of the virus may cause warts that are considered benign lesions and low or high grade intraepithelial lesions (LSIL/HSIL). Immunological system plays an important role in the resolution of infections. In this context, we highlight the chemokines, which are important regulators in the development of viral infections and inflammation. Among which CXCL12 stands out, due to its pro-inflammatory features, acting as chemoattractant recruiting immune cells. Several polymorphisms were identified in CXCL12 gene including rs1801157 in the 3′-untranslated region, which is characterized by a substitution of a guanine for an adenine.
  
  Methods
  In this study, 195 women were classified as HPV non-infected and 169 as HPV-infected. HPV-DNA was detected by polymerase chain reaction (PCR) and the polymorphism was assessed in blood cells through restriction fragment length polymorphism analysis.
  
  Results
  HPV infection was more incident in women who had more than 4 sexual partners during lifetime (p = 0.007), among those who presented lower number of pregnancies (p = 0.017). HPV was more prevalent among allele A carriers confirmed by logistic regression analysis adjusted for several confounding factors [ORADJ = 4.985; CI95% (2.85–8.72), p < 0.001]. An association between allele A carriers and HSIL development (p = 0.003) was also observed.
  
  Conclusions
  In the present study, we demonstrated that CXCL12 rs1801157 is independently associated with HPV infection and exerts influence in HSIL development, suggesting it as a promising susceptibility biomarker for HPV infection and lesions development.
• 中文關鍵字：
• 英文關鍵字： CXCL12, rs1801157 polymorphism, HPV infection, Cervical lesion