• 作者： 洪其璧；謝榮添；柯錫津；詹榮弘；李志恆
• 作者服務機構： 行政院衛生署藥物食品檢驗局
• 中文摘要： 原由本實驗室發展之寒天凝膠電氣泳動及抗菌譜之抗生素系統分析法，經改良以增進其定性效果及敏感度，供試菌種增加為6種後已可區分不同類之抗生素，寒天凝膠之厚度，供試菌種帶之厚度，及樣品縫之?度等之最佳條件經定出而使本分析法之敏感度達到食品中抗生素殘留量之範圍，加上一次操作可處理多數量檢體之可行性及再顯性等條件一起評價，顯示利用本分析法以檢驗食品中殘留抗生素之可能性。
• 英文摘要： Systematic analysis of antibiotics via agar gel electrophoresis and antimicrobial spectrum, a method previously developed in our laboratory, was modified to increase its resolution power and sensitivity. Six test organisms were employed in the analysis system so that antibiotics of different chemical groups can be distinguished from each other. Optimal conditions of the height of agar gel and test organism strip, and the width of sample slit were determined and modifications were made which enable the analysis of antibiotics at the concentration levels of food residues. Other features of the analysis system such as feasibility for handling large number of samples in a single procedure and reproducibility were together discussed so as to evaluate its candidacy for detecting residual antibiotics in foods.
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