- 作者: 林萬寅,葉慧君,許文東
- 作者服務機構: Department of Chemistry, National Taiwan University, Taipei 106, Taiwan, R.O.C.
- 中文摘要: Chemiluminescence (CL) from the cobalt(II)-catalyzed oxidation of luminol with hydrogen peroxide was dramatically enhanced by the presence of carbonate. The CL signal increases by several orders of magnitude over a wide range of concentrations of Co(II), luminol, or hydrogen peroxide. A limit of detection of 10-12 M for Co(II) and luminol and 10-8 M for hydrogen peroxide can be achieved. The CL emission spectrum exhibits a maximum at 425 nm, indicating that the excited 3-aminophthalate is the emitting species. ESR spin-trapping experiments revealed a large increase in the production of hydroxyl and carbonate radicals by the presence of carbonate, which is responsible for the enormous CL enhancement. Uric acid, ascorbic acid, acetaminophen, and p-hydroxyphenyl acetic acid are capable of scavenging the radicals, thereby inhibiting the CL emission. The inhibition of CL intensity can be used to determine these substances at the sub-micromolar level.
- 英文摘要: --
- 中文關鍵字: Chemiluminescence; Stopped-flow; Co(II)-Luminol-H2O2; Carbonate.
- 英文關鍵字: --