- 作者: Lei-Miao Yin; Gong-Hao Jiang; Yu Wang, Yan Wang; Yan-Yan Liu; Wei-Rong Jin; Yu-Dong Xu; Qing-Hua Zhang; Yong-Qing Yang
- 作者服務機構: Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai, China
- 中文摘要: --
- 英文摘要:
Background : Asthma has become an important public health issue and approximately 300 million
people have suffered from the disease worldwide. Nowadays, the use of acupuncture
in asthma is increasing. This study intended to systematically analyze and compare
the gene expression profiles between the asthmatic and acupuncture-treated asthmatic
rat lung, and tried to gain insight into the molecular mechanism underlying the early
airway response (EAR) phase of asthma treated by acupuncture.
Methods :
Four tag libraries of serial analysis of gene expression (SAGE) were established from
lung tissues of control rats (CK), asthmatic rats (AS), asthmatic rats treated by
acupuncture (ASAC), and control rats treated by acupuncture (CKAC). Bioinformatic
analyses were carried out by using the methods including unsupervised hierarchical
clustering, functional annotation tool of the database for annotation, visualization, and
integrated discovery (DAVID), gene ontology (GO) tree machine, and Kyoto
encyclopedia of genes and genomes (KEGG) pathway analysis.
Results :
There were totally 186 differentially expressed tags (P < 0.05, PCK/AS) between the
libraries of CK and AS, 130 differentially expressed tags between libraries of
AS/ASAC (P < 0.05, PAS/ASAC), and 144 differentially expressed tags between
libraries of CK/CKAC (P < 0.05, P CK/CKAC). The gene expression profiles of AS and
ASAC were more similar than other libraries via unsupervised SAGE clustering. By
comparison of PCK/AS and PAS/ASAC, the DAVID genes functional classification was
found to be changed from “immune response” to “response to steroid hormone
stimulus”, and the GO term “antigen processing and presentation of peptide antigen”
disappeared in PAS/ASAC. Totally 3 same KEGG pathways were found among the three
groups. Moreover, 21 specific tags of the acupuncture in treating asthma were
detected using Venn diagrams.
Conclusion :
Our SAGE research indicates that the gene expression profile of the EAR phase of
asthma could be effectively and specifically regulated by acupuncture, which suggests
that the gene expression of immune response and steroid hormone may play an
important role in the treatment. - 中文關鍵字: --
- 英文關鍵字: --