- 作者: Yong-Han Hong; Wen-Wan Chao; Miaw-Ling Chen; Bi-Fong Lin
- 作者服務機構: Department of Biochemical Science and Technology, Institute of Microbiology and Biochemistry, College of Life Science, National Taiwan University, Taipei, Taiwan; R.O.C.
- 中文摘要: --
- 英文摘要:
This study aimed to investigate if food components that exert anti-inflammatory
effects may be used for inflammatory disorders by examining alfalfa sprout ethyl
acetate extract (ASEA). The cytokine profile and life span of BALB/c mice with acute
inflammation after intra-peritoneal (ip) injection of 15 mg/kg BW lipopolysaccharide
(LPS) were determined. The results showed that the life span of LPS-induced
inflammatory mice were negatively correlated with serum levels of TNF-α, IL-6, and
IL-1β at 9 hr after LPS-injection, which indicated that suppressing these cytokines in
the late phase of inflammation may be beneficial for survival. The in vitro experiment
then showed that ASEA significantly reduced IL-6 and IL-1β production and the
NF-κB trans-activation activity of mitogen-stimulated RAW264.7 cells. To further
evaluate the anti-inflammatory effects of ASEA in vivo, BALB/c mice were tube-fed
with 25 mg ASEA/kg BW/day in 50 μl sunflower oil, while the control and PDTC
(pyrrolidine dithiocarbamate, an anti-inflammatory agent) groups were tube-fed with
50 μl sunflower oil/day only. After one week of tube-feeding, the PDTC group was
injected with 50 mg/kg BW PDTC and one hour later, all of the mice were injected
with 15 mg/kg BW LPS. The results showed that the ASEA and PDTC groups had
significantly lower serum TNF-α, IL-6, and IL-1β levels at 9 hr after LPS challenge,
and significantly higher survival rates than the control group. This study suggests that ASEA supplementation can suppress the production of pro-inflammatory cytokines
and alleviate acute inflammatory hazards. - 中文關鍵字: --
- 英文關鍵字: --