• 作者： 陳文輝; 劉明欽
• 作者服務機構： 臺灣糖業研究所
• 中文摘要： 應用酵素分離之甘蔗嫩葉片原生質體，經Carbol fucbsin染色、鏡檢，發現約有7％為多核原生質體。多核原生質體多數含有二到四個核，但含有十一個核之原生質體亦偶而可見。這些原生質體可能由於原生質體在分離過程中自然融合所導致。多核原生質體經一天培養可發生核融合，經二天培養可進行核分裂，核分裂可能數個細胞核同時進行或者不同時進行。剛分離之具有生機之原生質體用螢光染色法證明不具有細胞壁，經二十四小時培養，則細胞增大，延長，再生成細胞壁。經二到五天的培養開始第一次細胞分裂，繼續培養二十一天，少數細胞能繼續分裂形成細胞團。培養基內添加植物荷爾蒙和氯化鈣，或使用多種醣類當滲透壓穩定劑，可增加細胞壁之生成率及促進細胞分裂。芽胞分裂現象常在培養中被發現。
• 英文摘要： Viable protoplasts were readily obtainedfrom sugarcane young leaves after 2 hoursenzyme treatment. About 7% of the freshlyisolated protoplasts had more than one nucleusas shown by the staining with carbol fuchsin.Most of the multinucleates contained 2 to 4nuclei, but up to 11 nuclei were sometimesobserved. This leads to a postulation that themain source of the multinucleates may be aconsequence of a spontaneous fusion duringprotoplast isolation. Nuclear fusion was seenwhen the polynuclear protoplasts were culturedin the medium. Both synchronized and unsyn-chronized nuclear division in the multinucleateswere going on within 48 hours of culturing.Immediately after isolation, the viable proto-plasts showed no wall existed as evidenced byno fluorescence occuring when staining withCalcoflour White. After 24 hours culturing, newcell wall would be formed as demonstrated bythe occuring of fluorescence or as proved byplasmolyzing method. The protoplasts in hang-ing drop culture became oval in shape andunderwent the first cell division within 2 to 5days. A cell cluster would be formed after 3-week culturing. Both plant growth regulatorsand CaCl, were required for cell wall regenera-tion and cell division. The percentage ofprotoplasts undergoing cell wall regenerationand cell division was increased when themedium supplemented with mixture of sugarsas osmotic stabilizer was used. The phenomenonof "budding" was seen in many protoplasts.
• 中文關鍵字： --
• 英文關鍵字： --