- 作者: 劉高家秀;蘇清鎰;李文山
- 作者服務機構: 國立師範大學化學系
- 中文摘要:
利用派來克斯玻璃及單心銅線做成延長器,使用插入電子束撞擊法及液態、固態介質單離之技術可成功地測到核酸
成分之分子及類分子離子譜線。對核鹼類,核?類及具有保護基之去氧核?類化合物而言,這些質譜的品質及斷裂型式
與其他緩和游離法所得者類似。靈敏度對烏糞嘌呤測定之結果為5 × 克,對2'-去氧鳥糞核?為5 × 克。C - 英文摘要: An extremely simple and effective modification has been developed on the Jeol MSD-300 massspectrometer. Using an extended probe and the in-beam El method, together with liquid and solid ma-trices, molecular ions and/or quasimolecular ions of nucleic acid components can be obtained. This methodis excellent for nucleic bases, nucleosides, and protected nucleosides. The qualities and fragmentation pat-terns are comparable to those obtained by other soft ionization methods. The detection limits have beendetermined as 5 x for guanine and 5 x for 2'-deoxyguanosine.
- 中文關鍵字: in-beam El of nucleic acid components
- 英文關鍵字: --