- 作者: 胡秋芬,吳獻花,楊光宇,董章杰,尹家元
- 作者服務機構: Department of Chemistry, Yunnan University, Kunming, 650091, P. R. China
- 中文摘要: A new method for the simultaneous determination of five transition metal ions in water and food by rapid high-performance liquid chromatography was developed. The cobalt, nickel, copper, zinc and vanadium ions were pre-column derivatized with 2-(2-quinolinylazo)-4-methyl-1,3-dihydroxidebenzene (QAMDHB) to form colored chelates, then the Co-QAMDHB, Ni-QAMDHB, Cu-QAMDHB, Zn-QAMDHB and V-QAMDHB chelates were enriched by solid phase extraction with a C18 cartridge. The enrichment factor of 50 was achieved by eluting the retained chelates from the cartridge with tetrahydrofuran (THF). These chelates were separated on a ZORBAX Stable Bound rapid analysis column (4.6 ’ 50 mm, 1.8 mm) with 68% methanol (containing 0.1% of acetic acid and 0.1% of CTMAB) as mobile phase at a flow rate of 2.0 mL/min and detected with a photodiode array detector from 450~600 nm. The Co-QAMDHB, Ni-QAMDHB, Cu-QAMDHB, Zn-QAMDHB and V-QAMDHB chelates were separated completely within 2.0 min. The detection limits of cobalt, nickel, copper, zinc and vanadium are 2 ng/L, 1.5 ng/L, 2 ng/L, 3 ng/L, and 3 ng/L, respectively, in the original samples. This method was applied to the determination of the five transition metal ions in water and food samples with good results.
- 英文摘要: --
- 中文關鍵字: Rapid high-performance liquid chromatography; 2-(2-quinolinylazo)-4-methyl-1,3-dihydroxidebenzene; Transition metal ions.
- 英文關鍵字: --