- 作者: Zahra Hajihassan; Azra Rabbani-Chadegani
- 作者服務機構: Department of Biochemistry, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
- 中文摘要: --
- 英文摘要:
Mitoxantrone is a potent antitumor drug, widely used in the treatment of various
cancers. In the present study, we have investigated and compared the affinity of
anticancer drug, mitoxantrone, to EDTA-soluble chromatin (SE-chromatin), DNA and
histones employing UV/Vis, fluorescence, CD spectroscopy, gel electrophoresis and
equilibrium dialysis techniques. The results showed that the interaction of
mitoxantrone with SE-chromatin proceeds into compaction/aggregation as revealed
by reduction in the absorbencies at 608 and 260 nm (hypochromicity) and
disappearance of both histones and DNA on the gels. Mitoxantrone interacts strongly
with histone proteins in solution making structural changes in the molecule as shown
by CD and fluorescence analysis. The binding isotherms demonstrate a positive
cooperative binding pattern for the chromatin- mitoxantrone interaction. It is
suggested higher binding affinity of mitoxantrone to chromatin compared to DNA
implying that the histone proteins may play an important role in the chromatinmitoxantrone
interaction process. - 中文關鍵字: --
- 英文關鍵字: --