• 作者： Chen, Hanping Peter; Yang, Chin-Ping; Marmarelis, Vasilis Z.
• 中文摘要： 環狀糊精糖轉移(CGTase)用於由澱粉生產環狀糊精(CD)之酵素。在反應中還元糖不但為其主要副產物且妨礙CD之生成。本實驗以葡萄糖代替還元糖觀察CD生產之影響。當葡萄糖之濃度很低時妨礙效果可以忽略可用 Michaelis -Menten model解釋其反應, 並可由 Lineweaver-Burk plot求得V/sub max/與 K/sub m/。當葡萄糖之濃度相對增加時, CD生成效果明顯地被抑制, 本文先假定它是一種常見之物理性抑制反應---混合型抑制反應來解釋其反應和試求其參數。然後說明葡萄糖在反應中不但會減少CD之產率且會使CD分解, 是一種酵素之化學反應, 其反應動力將待進一步研究。
• 英文摘要： CGTase (cyclodextrin glycosyltransferase) was used as an enzyme to produce CD (cyclodextrin). Reducing sugar was one of the main by-products during the reaction and showed an inhibitory effect on the formation of CD. In the experiment, glucose was used to replace reducing sugar. When the concentration of glucose is very low, its effect can be neglected and a Michaelis-Menten model used to describe the reaction. The parameters V/sub max/ (maximum reaction rate) and K/sub m/ (Michaelis-Menten constant) were obtained by a Lineweaver-Burke plot. When the concentration of equivalent glucose increases, its inhibitory effect can be observed. The "mixed-type inhibitory reaction" illustrated that the inhibitory effect was caused by high glucose concentrations. The reaction parameters can be found again by the graphic method. Glucose not only prevented the formation of CD but also combined with CGTase to destroy the produced CD. This degradation reaction is one kind of enzymatic reaction. The kinetics of the reaction leading to the production of CD will be investigated.
• 中文關鍵字： 環狀糊精; 起始速率; 抑制效應; Michaelis-menten速率常數; Lineweaver-Burk圖; 分解; 葡萄糖
• 英文關鍵字： Cyclodextrin; Initial Rate; Inhibitory Effect; Michaelis-Menten Rate Constant; Lineweaver-Burk Plot; Degradation; Glucose