- 作者: Kukhoun Jeong; Yong-Cheol Shin; Sunju Park; Jeong-Su Park; Namil Kim; Jae-Young Um; Hoyeon Go; Seungho Sun; Sundong Lee; Wansu Park; Youkyung Choi; Yunkyung Song; Gyungjun Kim; Chanyong Jeon; Jonghyeong Park; Keysang Lee; Oksun Bang; Seong-Gyu Ko
- 作者服務機構: Center for Clinical Research and Genomics, Kyung Hee University, Seoul, Korea
- 中文摘要: --
- 英文摘要:
Aging is a progressive process related to the accumulation of oxidative damage and
neuroinflammation. We tried to find the anti-amnesic effect of the Scutellaria baicalens
Georgia (SBG) ethanol extract and its major ingredients. The antioxidative effect of SBG
on the mice model with memory impairment induced by chronic injection of D-galactose
and sodium nitrate was studied. The Y-maze test was used to evaluate the learning and
memory function of mice. The activities of superoxide dismutase, catalase and the content
of malondialdehyde in brain tissue were used for the antioxidation activities.
Neuropathological alteration and expression of bcl-2 protein were investigated in the
hippocampus by immunohistochemical staining. ROS, neuroinflammation and apoptosis
related molecules expression such as Cox-2, iNOS, procaspase-3, cleaved caspase-3, 8 and
9, bcl-2 and bax protein and the products of iNOS and Cox-2, NO, PGE2, were studied
using LPS-activated Raw 264.7 cells and microglia BV2 cells. The cognition of mice was
significantly improved by the treatment of baicalein and 50 and 100mg/kg of SBG in Ymaze
test. Both SBG groups showed strong antioxidation, antiinflammation effects with
significantly decreased iNOS and Cox-2 expression, NO and PGE2 production, increased
bcl-2 and decreased bax and cleaved caspase-3 protein expression in LPS induced Raw
264.7 and BV2 cells. We also found that apoptotic pathway was caused by the intrinsic
mitochondrial pathway with the decreased cleaved caspase-9 and unchanged cleaved
caspase-8 expression. These findings suggest that SBG, especially high dose, 100mg/kg,
improved the memory impairments significantly and showed antioxidation,
antiinflammation and intrinsic caspase-mediated apoptosis effects. - 中文關鍵字: --
- 英文關鍵字: --