- 作者: 阮忠清; 呂勝春
- 作者服務機構: 中央研究院生物化學研究所; 國立臺灣大學醫學院臨床醫學研究所
- 中文摘要: 將B型肝炎表面抗原的單源抗體結合在溴化氰活化之Sepharose-4B上,做成免疫吸附親和性管柱(immunosorbentaffinity column)發展出一種經濟有效又快速簡便分離B型肝炎表面抗原的方法,配合氯化銫濃度梯級超高速離心法,可得精純的B型肝炎表面抗原。 “e”抗原陽性的血漿,含有高力價的B型肝炎表面抗原,只需經2.77%氯化鈣溶液將纖維蛋白原(fibrinogen)沈澱,得到的血清立即通過親和性管柱,以3M NaSCN洗出HBs回收的HBs與傳統數個步驟isopycnic&zonal ultracentri-fugation分離者比較具有同樣純度,其回收率為70%以上。 在經濟效益上,親和性管柱可以重複使用,在本論文中,該管柱連續分離HBs 30次以上,歷經二個月,全無任何劣變現象。
- 英文摘要: Using immobilized monoclonal antibodies (anti-HBs) to hepatitis B surface antigen (HBs) as an im-munosorbent affinity column, a simple and effective procedure for HBs purification has been developed. A serum sample containing high titer HBs (i.e. HBe-positive serum) is passed through the columnwithout prior treatment. The HBs is further purified by cesium chloride gradient ultracentrifugation. Therecovery of HBs is greater than 70% while the purity is very comparable to those obtained from severalisopycnic and rate zonal ultracentrifugation procedures (Dreesman et al., 1972). Over a period of twomonths, the column was used repeatedly for thirty cycles without any noticeable deterioration.
- 中文關鍵字: HBs; monoclonal antibodies; affinity column
- 英文關鍵字: --