- 作者: Shao Hua Chen Raymond Tak Fai Cheung
- 作者服務機構: Division of Neurology, University Department of Medicine, University of Hong Kong, Hong Kong
- 中文摘要: --
- 英文摘要: In this in vitro study, we investigated the influence ofneuropeptide Y (NPY) Y1 receptor activation or inhibitionon the viability of cultured neuronal or glial cells follow-ing oxygen glucose deprivation (OGD). Viability of cul-tured cells was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. When compared tothe vehicle-treated control group, treatment with NPY or[Leu31,Pro34]-NPY (Y1 agonist) reduced viability of cul-tured SK-N-MC (Y1-expressing) human neuronal cells at24 h after 1 h of OGD, while BIBP3226 (Y1 antagonist)improved viability. Except at the highest concentration ofNPY used in the study, treatment with NPY or NPY3-36(Y2 agonist) did not influence viability of culturedSH-SY5Y (Y2-expressing) human neuronal cells at 24 hafter 1 h of OGD. In addition, treatment with NPY,[Leu31,Pro34]-NPY, NPY3-36, or BIBP3226 did not affectviability of cultured primary astrocytes at 24 h after 4 h ofOGD. The present results agree with those of a recent invivo study. Activation of NPY-Y1 receptors may mediateischemic pathophysiological processes, and inhibitingthe Y1 receptors may be protective. The combination ofOGD and cultured neuronal cells may be useful in futurestudies on the neuroprotective and harmful mechanismsof NPY-Y1 receptor inhibition and activation during isch-emia, respectively.
- 中文關鍵字: --
- 英文關鍵字: --