- 作者: Yuanhong Liang; Shuguang Lin; Yan Zhou; Jinming Wang; Xiyong Yu
- 作者服務機構: 1 Research Center of Medical Sciences, Guangdong Provincial people's Hospital, Guangdong Cardiovascular Institute, 96 Dongchuan Road, Guangzhou, 510100 China; ; 2 Department of Emergency, Renmin hospital of Wuhan University, 238 JieFang Road, Wuhan, 430060China;; 3 Department of Cardiology, Renmin hospital of Wuhan University, Wuhan, China
- 中文摘要: --
- 英文摘要: Gene therapy has been applied to reduce blood pressure in rats. However, little is known about the effects of gene therapy on both blood pressure and left ventricular (LV) remodeling. This study was designed to compare the antihypertensive effect of β1 adrenergic receptor antisense oligodeoxynucleotides (β1-AS-ODN) by delivery with the different charge ratios cationic liposomes DOTAP/DOPE and its impact on the LV remodeling in rats with 2-Kidney, 1-Clip (2K1C) Hypertension. Five charge ratios of liposome/ ODN were tested in 2K1C rats. There was hypertension, cardiac dysfunction, LV hypertrophy and LV collagen deposition in 2K1C rats. On the basis of the magnitude and duration of hypotension, 2.0 was determined to be the optimal charge ratio, which decreased blood pressure by up to 39 mm Hg for 27 days. β1-AS-ODN preserved cardiac function and inhibited LV mass and LV interstitial collagen deposition. In conclusion, cationic liposomes DOTAP/DOPE improve the antihypertensive effects of β1-AS-ODN in renovascular hypertension and 2.0 were determined to be the optimal charge ratio. This study demonstrated that cardiac BrAR played a key role in LV remodeling and β1-AS-ODN ameliorates cardiac dysfunction and LV remodeling.
- 中文關鍵字: --
- 英文關鍵字: gene therapy, hemodynamics, hypertension, remodeling