- 作者: Shui Qing Ye a, David C. Usher b, Li Q. Zhang c
- 作者服務機構: a Division of Pulmonary and Critical Care Medicine, Johns Hopkins University school of Medicine, Baltimore, Md., b Department of Biological Science, University of Delaware, Newark, Del., and c Department of surgery, University of Maryland, Baltimore, Md., USA
- 中文摘要: --
- 英文摘要: Until recently, the approach to understanding the molec-ular basis of complex syndromes such as cancer, coro-nary artery disease, and diabetes was to study thebehavior of individual genes. However, it is generallyrecognized that expression of a number of genes is coor-dinated both spatially and temporally and that this coor-dination changes during the development and progres-sion of diseases. Newly developed functional genomicapproaches, such as serial analysis of gene expression(SAGE) and DNA microarrays have enabled researchersto determine the expression pattern of thousands ofgenes simultaneously. One attractive feature of SAGEcompared to microarrays is its ability to quantify geneexpression without prior sequence information or infor-mation about genes that are thought to be expressed.SAGE has been successfully applied to the gene expres-sion profiling of a number of human diseases. In thisreview, we will first discuss SAGE technique and con-trast it to microarray. We will then highlight new biologi-cal insights that have emerged from its application to thestudy of human diseases.
- 中文關鍵字: --
- 英文關鍵字: Gene expression profiling, Human diseases, RNA, Serial analysis of gene expression