• 作者： 王長君; 劉江川; 張白毅
• 作者服務機構： 國防醫學院生物及解剖學; 美國德州大學蓋氏醫學分校人類生物化學及遺傳學系
• 中文摘要： Sprague-Dawley大白鼠以黃麴毒素B 單劑量（3 mg／kg）腹腔注射處理後經3，6，12，24小時及1，2，5週後分別犧牲之
  ，取其肝臟作電子顯微鏡微細構造及細胞化學三種標示?，酸性燐、酸脂?（AcPase）、胺焦燐、酸脂?（TPPase）及葡萄
  糖-6-燐、酸脂?（G6Pase）活性位置變化之觀察。3-6小時後，核仁產生凝聚現象，核仁纖維部及顆粒部網狀結構消失
  。平行排列之顆粒內質網散置而平滑內質網增多。 12-24小時後，核仁，內質網之破壞更形顯著；多核糖體分散，游離
  之核糖體增加。平行內質網排成渦狀圍於脂小球周圍，亦見於細胞質塊中；皆顯現G6Pase活性反應。AcPase活性見於
  次級溶體中，細胞質塊中則無AcPase活性反應。TPPase活性見於膽小管及高基氏體中。1-2週後，多數細胞開始恢復正
  常，細胞質塊消失，平行排列之顆粒內質網亦再行出現。5週後，核仁呈現正常形態。線粒體則始終維持正常狀況。結
  論如下： 1．肝細胞在此毒素劑量下，於處理12-24小時後受到最大傷害，但核仁早於黃麴毒素處理3小時或更早，則已
  發生變化。2．肝細胞對此毒素呈現不同程度的反應，抵抗力及恢復能力。但單劑量處理仍無以導致癌細胞之產生。3．三
  種標示?在實驗過程中TPPase活性雖有減少之趨勢，但三者皆始終存在。
  t
• 英文摘要： Young male SPrague一Dawley rats were injeeted intraPeritoneally with a single dose of afiatoxinB (AFB , 3 mg/kg). At3, 6, 12, and 24 hours and l, 2, and 5 weeks, the rats were killed and liver sampleswere taken for examination　of sequential ultrastructural changes and localization of acid phosphatase(AcPase), thiamine pyrophosphatase(Tppase)and glucose-6-phosphatase(G6Pase) activity· At 3-6 hrs ofAFB treatment, the nucleoli became compacted and the network forms of nucleolonema disappeared·Parallel arrays of rough ER encountered in normal liver cells became deranged. Smooth ER increased toform groups of SER anastomosis or vesicles near the golgi area. At 12-24 hours, disruptions of nucleoli, ER systems, and polysomes became more evident. Parallel arrays of ER membranes, forming whorls inthe cytoplasm as well as in the cytoplasmic patches(CP), were G6Pase-positive, although the CP wereAcPase-negative. The TPPase reaction in bile canaliculi was frequently diminished, but was present insome measure in the Golgi saccules. By l-2 weeks, most of the injured cells had recovered gradually. TheCP disappeared and parallel arrays of RER were observed again in most parenchymal cells. At 5 weeks, theappearance of the nucleoli was normai, as was that of the other organelles. We concluded that the hepaticparenchymal cells had serious lesions at 12 and 24 hours of AFB treatment and then recovered nonsyn-chronously. The response, resistance, and ability to recover from the toxicity of AFB varied among theparenchymal cells. The three marker enzymes persisted throughout all regimens of AFB treatment.
• 中文關鍵字： aflatoxin B1; acid phosphatase; thiamine pyrophosphatase; glucose-6-phosphatase
• 英文關鍵字： --