- 作者: Tai Chu Peng; Kuang Wen Liao; Hui Ling Lai; Yann Fen C. Chao; Fwu Mei Chang; Horng-Jyh Harn; Ru Ping Lee
- 作者服務機構: 1 Institute of Medical Sciences (TCP, HJH), Tzu Chi University, Hualien, Taiwan, R.O.C; ; 2 Department of Nursing (TCP), Tzu Chi College of Technology, Hualien, Taiwan, R.O.C;; 3 Department of Biological Science and Technology (KWL), National Chiao Tung University, Hsinchu, Taiwan, R.O.C; ; 4 Department of Nursing (HLL, RPL), Tzu Chi General Hospital, Hualien, Taiwan, R.O.C; ; 5 School of Nursing, National Yang Ming University (YFCC), Taipei, Taiwan, R.O.C; ; 6 Department of Nursing (FMC, RPL), Tzu Chi University, Hualien, Taiwan, R.O.C;; 7 Neuro-Medical Scientific Center (HJH, RPL), Tzu Chi Medical Center, Hualien, Taiwan, R.O.C
- 中文摘要: --
- 英文摘要: Hemorrhagic shock is a common cause of death in emergency rooms. Current animal models of hemorrhage encounter a major problem that the volume and the rate of blood loss cannot be controlled. In addition, the use of anesthesia obscures physiological responses. Our experiments were designed to establish an animal model based on the clinical situation for studying hemorrhagic shock. Hemorrhagic shock was induced by withdrawing blood from a femoral arterial catheter. The blood volume withdrawn was 40% of the total blood volume for group 1 and 30% for group 2 and 3. Group 3 was anesthetized with sodium pentobarbital (25 mg/kg, i.v.) at the beginning of blood withdrawal. Our data showed that the survival rate was 87.5% at 48 h in the conscious group and 0% at 9 h in anesthetic group after hemorrhage. The levels of mean arterial pressure, heart rate, white blood count, TNF-α, IL1-β, CPK, and LDH after blood withdrawal in the anesthetic group were generally lower than those in conscious groups. These results indicated that anesthetics significantly affected the physiology of experimental animals. The conscious, unrestrained and cumulative volume-controlled hemorrhagic shock model was a good experimental model to investigate the physical phenomenon without anesthetic interfernce.
- 中文關鍵字: --
- 英文關鍵字: animal model, conscious rats, cumulative hemorrhage, lactic acid, organ injury, unrestrained