- 作者: Ching-Wen Chen; Chin-San Liu; Ing-Ming Chiu; Shih-Cheng Shen; Hung-Chuan Pan; Kun-Hsiung Lee; Shinn-Zong Lin; Hong-Lin Su
- 作者服務機構: Department of Life Sciences, National Chung-Hsing University, Taichung, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
Background :
Neural induction is a complex process and the detailed mechanism of FGF-induced
neurogenesis remains unclear.
Methods :
By using a serum-free neural induction method, we showed that FGF1
dose-dependently promoted the induction of Sox1/N-cadherin/nestin triple positive
cells, which represent primitive neuroblasts, from mouse embryonic stem (ES) cells.
Results :
We demonstrated that FGF1, FGF2, and FGF4, but not FGF8b, enhanced this
neurogenesis. Especially, FGF-enhanced neurogenesis is not mediated through the
rescue of the apoptosis or the enhancement of the proliferation of Sox1+ cells. We
further indicated that the inactivation of c-Jun N-terminal kinase-1 (JNK-1) and
extracellular signal-related kinase-2 (ERK-2), but not p38 mitogen-activated protein
kinase (MAPK), inhibited the neural formation through the inhibition of ES
differentiation, but not through the formation of endomesodermal cells.
Conclusions :
These lines of evidence delineated the roles of FGF downstream signals in the early
neural differentiation of ES cells. - 中文關鍵字: --
- 英文關鍵字: --