- 作者: Chia-Chann Shiue, Shu-Yu Lin and Chuen-Ying Liu
- 作者服務機構: Department of Chemistry, National Taiwan University, Taipei, Taiwan, R.O.C.
- 中文摘要: A novel mixed-mode stationary phase for open tubular capillary electrochromatography (CEC) was prepared. With 3-aminopropyltrimethoxysilane as the spacer, the chemical reagent in conjunction with the fullerene played an important role in the electrochromatographic separation of the plant phenols. The fullerene C60-based column has the properties of both hydrophilic and hydrophobic interaction sites and provides a positively charged capillary surface responsible for the reversed electroosmotic flow (EOF) in the column during CEC operation. Comparative studies for the fullerene-bonded phase, the intermediate phase with amino group and the bare fused-silica were carried out. Under the conditions of acetate buffer (20 mM, pH 5.0) and applied voltage of -15 kV, the separation of catechin, salicylic acid, myricetin, quercetin, gallic acid, caffeic acid and p-coumaric acid could be achieved. The proposed method was also applied to the analysis of plant phenols in grape juice.
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