- 作者: Terabe, Shigeru ; Ozaki, Hiroto ; Tanaka, Yoshihide
- 作者服務機構: Himeji Inst. of Technology, Kamigori, Hyogo, Japan
- 中文摘要: To extend the applicability of electrokinetic chromatography (EKC), two new types of pseudostationary phases have been introduced. A high-molecular surfactant, butyl acrylate/butyl methacrylate/methacrylic acid copolymer(BBMA) is employed as a micellar forming surfactant for micellar electrokinetic chromatography (MEKC). The critical micelle concentration of BBMA is essentially zero, which means the micellar concentration is constant irrespective of temperature and buffer. Some characteristic features of BBMA as the pseudostationary phase for MEKC is investigated in comparison with conventional ionic surfactants. Ovomucoid and avidin, which are proteins isolated from egg white, have been found to be useful chiral selectors in affinity EKC. A few examples of the separation of enantiomers with these proteins are shown.
- 英文摘要: --
- 中文關鍵字: Capillary Electrophoresis (Ce); Electrokinetic Chromatography (Ekc); Enantiomer; Ovomucoid; Avidin
- 英文關鍵字: 毛細管電泳;電動力層析法;雙掌異構物;卵擬黏蛋白;卵白素