- 作者: Low-Tone Ho; You-Chung Chou; Jau-Song Yu; Shiaw-Der Yang
- 作者服務機構: a Department of Medical Research and Education, Veterans General Hospital-Taipei, Taipei, b Graduate Institute of Medical Sciences,National Defense Medical Center, Taipei, c Department of Cell and Molecular Biology,Institute of Basic Medicine, Chang GungMedical College, Tao-Yuan, d Institute of Biomedical Sciences,National Tsing Hua University,Hsinchu, Taiwan
- 中文摘要: --
- 英文摘要: In this study, we investigate the effects of endothelin-1 (ET-1) and insulin on the cellular activity of protein kinase FA/glycogen synthase kinase-3α (kinase FA/GSK-3α) in rat adipocytes. The cellular activity of kinase FA/GSK-3α is inhibited to ~ 50% of control within 30 min when cells are treated with 1 nM ET-1 at 37°C; in addition, significant inhibition to ~ 60% of control is observed at as low as 1 pM ET-1. Conversely, ET-1 at concentrations up to 1 nM has no direct effect on purified kinase FA/GSK-3α in vitro. Immunoblot-ting analysis further reveals that the protein level of this kinase is not significantly changed when treated with 1 nM ET-1 for 30 min. Similar to ET-1, insulin as low as 10 nM can also induce inactivation of kinase FA/GSK-3α to ~ 50% of control in adipocytes when processed under identical conditions. Most importantly, when treated with both insulin and ET-1, the activity of kinase FA/GSK-3α can be decreased only to ~ 50% of control. Taken together, the results provide initial evidence that ET-1 and insulin may regulate this important multisubstrate/multifunctional protein kinase in a common signal
- 中文關鍵字: --
- 英文關鍵字: Endothelin-1 ; Insulin; Protein Kinase Fa/GSK-3α; MAP kinase signaling pathway ; Rat adipocytes