- 作者: 壽廉; 楊錫銘; 張仲明; Robert A. Good
- 作者服務機構: 國防醫學院微生物及免疫學科; 三軍總醫院臨床免疫學研究室; 陽明醫學院微生物及免疫學研究所; Oklahoma Medical Research Foundation
- 中文摘要: 研究PEG沈澱法測定血中免疫複合物,在4% PEG的濃度下,發現最適合的沈澱酸鹼度為7.8。當血中免疫複合物濃度高時,回收率為73.9-101.3%,濃度低時回收率變化較大,為57.8-146.5%,重覆檢出率係數為0.5-9.5。實驗結果顯示此法用作測定血中免疫複合物的可靠性頗高。 經用4℃ PEG在上述相同實驗情況下,測定三種肝病-肝炎、肝癌、肝硬化、鏈球菌感染後腎絲球炎及SLE五類病人血中免疫複合物濃度,結果顯示除了治療後的腎絲球炎患者外,其他各類病人血中免疫複合物皆升高,此一結果顯示免疫複合物與許多疾病之間有密切的關係。
- 英文摘要: A method for detection and quantitation of circulating immune complexes using precipitation of thecomplexes by polyethylene glycol (PEG) has been reexamined to determine the influence of pH on therecovery and the reproducibility of the results. Results showed that the pH optimum for these determina-tions was 7.8. The recovery percentages range from 57.8-146.5% at lower immune complex concentrations,and from 73.9-101.3% at higher concentrations. The reproducibility of the method seems reasonably ac-ceptable with a percent coefficient of variation ranging from 0.5-9.5. This method for quantitation of cir-culating immune complexes by polyethylene glycol precipitation is consistent and relatively reliable. Usingthis method, the levels of circulating immune complexes in sera in patients with hepatitis, liver cirrhosis,hepatoma, acute post-streptococcal glomerulonephritis (before and after treatment) and systemic lupuserythematosus have been examined. The results showed that except the patients with treated acute post-streptococcal glomerulonephritis who had a similar amount of immune complexes with normal controls,the level of immune complexes in patients with other types of diseases were all higher than the control. Inaddition, the composition of IgG, IgA, IgM, C3 and C4 of the precipitable complexes in sera of patientswith three types of liver disease has been analyzed and demonstrated that the percentages of IgM werehigher than the normal control. However, C3 and C4 in hepatitis and liver cirrhosis patients were lowerthan those of the control.
- 中文關鍵字: circulating immune complexes; polyethylene glycol precipitation
- 英文關鍵字: --