- 作者: Lloyd A. Pereira, Melissa J. Churchill, Andrew G. Elefanty, Theo Gouskos, Paul F. Lambert Robert G. Ramsay, Nicholas J. Deacon
- 作者服務機構: Laboratory for Physiological Chemistry and Centre for Biomedical Genetics, University Medical Centre, Utrecht, The Netherlands, AIDS Molecular Biology Unit, National Centre for HIV Virology Research, The Macfarlane Burnet Centre for Medical Research, Fairfield, Vic., Division of Cancer and Haematology, The Walter and Eliza Hall Institute of Medical Research, PO Royal Melbourne Hospital, Parkville, Vic., c/-School of Medicine, Flinders University of south Australia, Bedford Park, S.A, and Peter MacCallum Cancer Institute, Standrew's Place, East Melbourne, Vic., Australia
- 中文摘要: --
- 英文摘要: context of a minichromosome assembled full-lengthLTR, mutation of region -320 to -281 increased basaland PMA-stimulated LTR activity. These findings sug-gest that this region is an important component of theHIV-1 LTR required for response todifferent cellular tran-scription factors.HIV-1 gene expression is regulated by the interplay oftranscription factors with multiple binding motifspresent within the U3, R and U5 regions of the long ter-minal repeat (LTR). Here we report novel DNA bindingcomplexes (termed 9a, 9b and 9c) between nuclear pro-teins from T-lymphoid and non-T-lymphoid cells and aregion of the U3 LTR between nucleotides (nts) -320 to-281 in the HIV strain HXB2. Complex 9b bound a motifpredicted to bind E-box or c-Myb proteins and a partiallyoverlapping dyad symmetrical motif, and included basichelix-loop-helix proteins (E12, E47 or ITF-1) but surpris-ingly not c-Myb. Complex 9c, which bound to a pair ofGATA sites, included GATA-3 and GATA-4 in Jurkat andMT-2 cells, respectively. We also demonstrate that thec-Myb/E-box and GATA sites form a bipartite motifrequired for the formation of complex 9a.Transienttransfection experiments with T cells revealed that in the
- 中文關鍵字: --
- 英文關鍵字: HIV-1 LTR promoter, c-Myb, Basic helix-loop-helix, GATA factor