- 作者: June L. Traicoff, James K.V. WIllson Sanford D. Markowitz
- 作者服務機構: Department of Molecular Biology and Microbiology , Case Western Reserve University , Ireland Cancer Center , University Hospitals of Cleveland , Howard Hughes Medical Institute , Cleveland , Ohio , USA
- 中文摘要: --
- 英文摘要: These data are the first evidence that expression of DCCtranscript can be silenced in colon adenoma cell linesand tissues. These data indicate that loss of DCC expres-sion occurs in some colon adenomas, but is insufficientto drive the adenoma to carcinoma progression.We examined the expression of the putative tumor sup-pressor gene deleted in colorectal carcinoma (DCC) inhuman colon adenoma tissues and celllines. One alleleof DCC is deleted in 70% of human colon carcinomas,and DCC expression is undetectable in 90% of colon car-cinoma cell lines. One DCC allele is also deleted in 50%of human colon adenomas, but results from proteinexpression studies have differed as to whether completeloss of DCC expression could occur in colon adenomas,or instead correlates with progression of colon adenomato carcinoma. To further examine the timing of DCCexpression loss in colon adenomas, we assayed DCCtranscript levels in adenoma cell lines and tissues. Wemeasured DCC expression by a sensitive assay usingSouthern blot detection of the RT-PCR-amplified DCCtranscript. DCC expression was negligible or greatlyreduced in 4 of 14 colon adenomas, including 2 of 2 ade-noma cell lines and 2 of 12 adenoma tissue samples.
- 中文關鍵字: --
- 英文關鍵字: Colon adenoma, Tumor progression, Deleted in colorectal carcinoma gene