• 作者： 馮濟敏
• 作者服務機構： 國立陽明醫學院生化學科
• 中文摘要： 氟酚納辛－瓊膠被合成來嘗試分離多巴胺激動劑及拮抗劑之結受體。當大腦striatal膜與氟酚納辛－瓊膠共同培育後造成H-spiroperidol（H-SPI）對膜結合能力之降低。H-SPI結合能力之降低並不是由於氟酚納辛從氟酚納辛－瓊膠上游離出來所造成。氟酚納辛－瓊膠對于帶有H-SPI結合體之膜的吸附能力遠大於帶有H－多巴胺結合體之膜。當Striatal膜與氟納辛－瓊膠共溶接，H-SPI對膜結合之密度降低到百分之29而H－多巴胺之結合密度卻不受影響。此外，多巴胺拮抗劑對于H-propylnorapomorphine與膜之結合之抑制能力也降低至少一百倍，這些結果顯示在striatal膜上存在至少兩種多巴胺接受體。
• 英文摘要： Separation of dopamine (DA) agonist and antagonist receptors was attempted by means of a cova-lently-bound fluphenazine-agarose (Flu-agarose). Incubation of striatal membranes with Flu-agarose result-ed in loss of H-spiroperidol (H-SPI) binding sites, while incubation with non-coupled agarose did notcause any change. The loss of H-SPI binding to the Flu-agarose treated membranes was not attributed tothe release of fluphenazine from Flu-agarose as justified by several criteria. Flu-agarose adsorbed moreeffectively striatal membranes with H-SPI binding sites than those with H-DA binding sites. Followingincubation of the membranes with Flu-agarose (2.5 ml beads/100 mg membrane protein), the density ofH-SPI binding sites in the resulting membranes was reduced to 29%, whereas the density of H-DA bindingsites to the same membranes was not changed. In addition, the potencies of DA antagonists to inhibit H-N-propylnorapomorphine binding to the membranes were decreased more than a hundred times, while thepotencies of DA agonists were little affected. These results suggest that in the striatal membranes exist atleast two populations of DA receptors.
• 中文關鍵字： dopamine; receptor; fluphenazine-agarose; affinity adsorbent
• 英文關鍵字： --