- 作者: Kenta Watanabe; Masato Tachibana; Suk Kim; Masahisa Watarai
- 作者服務機構: Department of Veterinary Public Health, Faculty of Agriculture, Yamaguchi University, Japan
- 中文摘要: --
- 英文摘要:
Background :
The uptake of abortion-inducing pathogens by trophoblast giant (TG) cells is a key event in
infectious abortion. However, little is known about phagocytic functions of TG cells
against the pathogens. Here we show that heat shock cognate protein 70 (Hsc70)
contributes to bacterial uptake by TG cells and the EEVD motif of Hsc70 plays an important
role in this.
Methods :
Brucella abortus and Listeria monocytogenes were used as the bacterial antigen in this study.
Recombinant proteins containing tetratricopeptide repeat (TPR) domains were constructed
and confirmation of the binding capacity to Hsc70 was assessed by ELISA. The
recombinant TPR proteins were used for investigation of the effect of TPR proteins on
bacterial uptake by TG cells and on pregnancy in mice.
Results :
The monoclonal antibody that inhibits bacterial uptake by TG cells reacted with the EEVD
motif of Hsc70. Bacterial TPR proteins bound to the C-terminal of Hsc70 through its
EEVD motif and this binding inhibited bacterial uptake by TG cells. Infectious abortion
was also prevented by blocking the EEVD motif of Hsc70.
Conclusions :
Our results demonstrate that surface located Hsc70 on TG cells mediates the uptake of
pathogenic bacteria and proteins containing the TPR domain inhibit the function of Hsc70 by
binding to its EEVD motif. These molecules may be useful in the development of methods
for preventing infectious abortion. - 中文關鍵字: --
- 英文關鍵字: --