- 作者: 洪其璧; Sarah A. bruce; 曹安邦
- 作者服務機構: 行政院衛生署藥物食品檢驗局; 約翰霍普金斯大學公共衛生學院生物物理組
- 中文摘要: 為便於將贅生性變異已建立之田鼠細胞有關數據引伸於人體細胞,本研究工作同時就敘利亞田鼠細胞與人體細胞進行三個方向之實驗,以求較佳之瞭解。在正常與腫瘤細胞之特性研究方面,人體與田鼠細胞相似之處在其腫瘤細胞都具停泊獨立性、形成微腫瘤球之懸浮生長性、及外來成瘤性,而正常細胞則無此等特性;且腫瘤細胞比其正常細胞具較短之細胞增倍期、較高之飽和密度、較高之集落形成效率、及較高之溶血活性。但人體與田鼠細胞亦在染色體數目變化、接觸敏感性、血清需求性、及形態變異性方面顯示不同之處。在使用微粒子培養於外來成瘤性試驗之可行性研究方面,微粒子技術似已因降低所需接種腫瘤細胞數量而提高腫瘤形成試驗之敏感度。在正常人體與田鼠細胞之體外變異方面,發現對田鼠細胞之最高形態變異效率為先以N-methyl-N'nitro-N-nitrosoguanidine繼以可能因干擾染色體體系而促進變異之Griseofulvin處理之處理組。然而此等處理對人體細胞卻仍未能產生變異。
- 英文摘要: Three approaches have been taken to study simultaneously Syrian hamster cells and human cells inorder to develop an extrapolation from the more established hamster system to human cells. On theCharacterization of normal cells in comparison to tumor cells, human tumor cells and hamster tumor cellsshowed similarity in displaying anchorage independence, growth in suspension as micro tumor spheroids,and xenotumorigenicity in contrast to their respective normal cells; in addition, these tumor cells exhibitedshorter population doubling time, higher saturation density, higher cloning efficiency, and higher fibrinoly-tic activity relative to their respective normal cell types. Other differences including ploidy change, contactinhibition on growth, serum requirement, and morphological transformation were also noted betweenhuman and hamster cells. On the application of microcarrier culture for a xenotumorigenicity test, themicrocarrier technique seemed to have enhanced the sensitivity by reducing the number of inoculatedtumor cells required for tumor formation. On the in vitro transformation of normal human and hamstercells, the highest efficiency of morphological transformation of hamster cells has been observed in thegroup treated with N-methyl-N'-nitro-N-nitrosoguanidine followed by griseofulvin which was employedto enhance the transformation by disturbing the chromosome apparatus. However, no evidence of trans-formation was observed in the treated human cells thus far.
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