• 作者： 謝天渝; 楊家福
• 作者服務機構： 高雄醫學院牙醫學系口空顎面外科
• 中文摘要： 口腔黏膜下纖維化症（OSF）的特徵是在口腔黏膜下堆積過多的膠原纖維。OSF是一種膠原病，也被認為是癌前病
  灶。
  在先前的研究，我們發現到OSF的膠原蛋白質含量比在正常口腔黏膜內的含量高。本研究的目的在於探討OSF的發
  生是否與膠原蛋白?活性之高低有關。從我們的研究可證明OSF的膠原蛋白?活性低於正常口腔黏膜者，兩者之間的差
  異有統計學上之意義。另外從膠原蛋白?分解膠原纖維的形式來看可知經過部份純化後的OSF膠原蛋自?是一種動物性
  膠原蛋白?，其分子量大約是68.0 kDa。
  各
• 英文摘要： Oral submucous fibrosis (OSF) is characterized by an abnormal accumulation of collagen fiber inoral submucosa. OSF is a collagen disease and is also regarded as a precancerous lesion. In the previous study, we discovered that the collagen content in oral mucosa of OSF is statisticallyhigher than in normal mucosa. This research examined the relationship between the fibrosis and collagenaseactivity. Collagenase activity was determined by using soiuoie C-giycine-labeled collagen (9813 cpm/200 g/tube) as a substrate in a solution incubated for 30 hours at 35℃. The results showed that the collagenaseactivity of the OSF was much lower than that of normal oral mucosa (65.23±19.49 units/g tissue innormal mucosa vs. 29.48±5.69 units/g tissue in OSF). Furthermore, the cleavage pattern revealed bySDS-Poiyacrylamid gel electrophoresis (SDS-PAGE) confirmed that the partially purified OSF collagenasewas likely to be typical mammalian collagenase, with a molecular weight of about 68.0 kDa.
• 中文關鍵字： collagenase activity; oral submucous fibrosis.
• 英文關鍵字： --