- 作者： 劉明欽; 葉惠心
- 作者服務機構： 臺灣糖業研究所育種系
- 中文摘要：
當甘蔗品種 F177 之癒合組織產生眾多的綠芽時，將其接種於含有1.17％氯化鈉之培養基上，作初步耐?篩選，不
耐鹽者不能生長，生長者即為耐鹽者。然後將耐鹽芽條個別選出並分開，臥接於含有0.94％及1.17％鹽份之培養基上，
一個月後從芽條基部長出旺盛的癒合組織，該組織亦同時分化出許多綠芽。從0.94％及1.17％鹽分篩選出的癒合組織分
別較正常癒合組織之水份潛勢低2倍及3倍，顯示此等癒合組織已具有抗鹽能力。源自經篩選過的芽條之癒合組織較未
經篩選過的芽條之癒合組織具有較高的芽條分化率，前者為40％，後者為21.5％。然後將此耐鹽芽條培養長大，並移植
於含鹽土壤中，其鹽份濃度為10個電導度，是屬於重鹽土類。3個月後，分析其葉片之各種離子含量。結果顯示：選出
的耐鹽植株較在正常土壤中生長的甘蔗吸收較多的氯及鈉離子。雖然該等離子有毒性，但不影響其正常生長，顯示用本
方法選出的甘蔗已具有適應重鹽土的能力，也是一個用組織培養方法能選出耐鹽甘蔗的實際例證。 - 英文摘要： The differentiated shoot-buds of sugarcane (Saccharum sp. hybrid) cv. F177 were subjected to pre-selection on 1.17%-NaCl medium. The survived shoots were laid down on MS medium with either 0.94% or1.17% NaCl. Callus mass was re-produced at the base of the survived shoots and concomitantly some of thecalli re-differentiated into shoots. The 0.94%-NaCl-selected callus was twice lower while the 1.17%-NaCl-selected callus was thrice lower in water potential than the normal callus. The NaCl-pre-selected callus gavehigher differentiation rate (40%) than the non-pre-selected callus (21.5%). The NaCl-tolerant shoots weregrown to a certain height and then transplanted into soil with salt strength in 10 millimhos/cm of electricconductivity. After three months growth, the NaCl-selected plant was found to accumulate more Na andCl ions than the normal plant without affecting its growth vigor. All these characteristics indicate that byusing this differentiated shoot-explant method, a salt-sensitive sugarcane plant can be shifted to a halophy-tic mode of salt tolerance.
- 中文關鍵字： --
- 英文關鍵字： --
