- 作者: Chun-Fen Chou; Hong-Wen Peng; Chung-Yih Wang; Ya-Ting Yang; Shou-Hwa Han
- 作者服務機構: Research Center for Immunology and Institute of Microbiology and Immunology, National Yang-Ming University, Department of Medicine, Armed Forces Taoyuan General Hospital, Taoyuna, and Department of Microbiology and Immunoloy, National Defense Medical Center, and Cheng Hsin General Hospital, Taipei, Taiwna, ROC
- 中文摘要: --
- 英文摘要: The transcriptional regulation of the Fas ligand (FasL)gene in Jurkat cells was investigated. We demonstratedthat an Sp1 binding site, located between -280 and -275by relative to the translational start site (+1) of the FasLgene, was important for the transcription of the FasLgene by deletion and mutation analysis in Jurkat cellsafter phorbol 12-myristate 13-acetate (PMA) and iono-mycin treatment. Nuclear extract of Jurkat cells formedcomplexes with the oligonucleotides bearing the Sp1site within -280 to -275 of the FasL promoter. Apart fromthe constitutive complexes, a new complex was ob-served after PMA and ionomycin stimulation. Plasmidcontaining the Sp1 site sequence with site-directed mu-tation reduced the FasL promoter activity in driving theexpression of reporter luciferase gene expression intransfected Jurkat cells after PMA and ionomycin stimu-lation. The binding of activated Jurkat cell nuclear extractto the mutated Sp1 binding site of the FasL promoter wasablated. In addition, the oligomer containing the Sp1 siteof the FasL promoter could compete with oligomer withconserved Sp1 binding sequence in nuclear proteinbinding of activated Jurkat cells. The data presented inthis study suggest that the transactivation of the FasLpromoter via the Spl binding sequence (-280 to -275)involves the PMA- and ionomycin-induced expression ofthe FasL gene.
- 中文關鍵字: --
- 英文關鍵字: --