- 作者: Kang-mai Wu; Chang-jen Huang; Sheng-ping L. Hwang; Yu-sun Chang
- 作者服務機構: 1 Institute of Microbiology and Immunology, National Yang-Ming University, Shih-Pai 112, Taipei Taiwan; ; 2 Institute of Biological Chemistry, Academia S璯ica, Nankang, Taipei 11529, Taiwan; ; 3 Institute of Cellular and Organismic Biology, Academia S璯ica, Nankang, Taipei 11529, Taiwan; ; 4 Graduate Institute of Basic Medical Sciences, Chang Gung University, 259 Wen-Hwa 1st Road, Kwei-Shan, Taoyuan 333, Taiwan
- 中文摘要: --
- 英文摘要: We have identified a cDNA clone encoding BMP receptor-associated molecule 1 (BRAMl) from the zebrafish expressed sequence tag (EST) database. The 2606 bp full-length bram1 cDNA was cloned, and further confirmed by nucleotide sequencing. The zebrafish sequence encodes a protein of 195 amino acids with an evolutionarily conserved MYND domain, which displays ~98% homology with human and mouse BRAM1, and ~64% homology with C. elegans BRA-1 and BRA-2. The braml gene, composed of five exons and four introns, spans ~14 kb on linkage group 14 of the zebrafish genome. RT-PCR and whole mount in situ hybridization analyses disclosed that zebrafish BRAM1 is a maternal factor. The protein interacts directly with zebrafish BMP Receptor type IA, as observed from GST-pull down and co-immu-noprecipitation assays. Furthermore, cotransfection of zebrafish BRAMl with the corresponding BMP receptor resulted in down-regulation of BMP-mediated signaling. Our results collectively indicate that BRAM1 plays a biological role during zebrafish development.
- 中文關鍵字: --
- 英文關鍵字: BRAM1, BMPRIA, whole-mount in situ hybridization, real time RT-PCR, zebrafish, BMP signaling