- 作者: Shu-Chun LIN, Kuo-Wei Chang, Che-Shoa Chang;Sin-Yuen Yu;Shou-Yee Chao;Yong-Kie Wong
- 中文摘要: This study identified that the carcinogenesis of hamster buccal pouch (HBP) induced by 7, 12-dimethyl-benz[a]anthracene (DMBA) was greatly enhanced (18 folds) by a combination treatment with Taiwanese betel quid (BQ) extract. A new cell line, HCDB-1, has been established from induced carcinomas. The cultured monolayer cells were epithelioid in shape with irregular nuclei. They demonstrated abundant cytokeratin and tonofilaments; however, ultrastructural well-organized desmosomes were lacking. The HCDB-1 cell exhibited population doubling in 19 h and was highly tumorigenic in nude mice. A C.arrlr.T transition at codon 141 (Ala to Val) of the p53 gene was detected in this cell. This mutation is equivalent to a specific temperature-sensitive mouse p53Ala135Val mutant that causes transformation by shifting to 37.5.degree.C. HCDB-1 is the first cell line established from the HBP model of oral carcinogenesis induced by DMBA/Taiwanese BQ extract. It might be valuable for exploring the molecular pathogenesis of oral cancer.
- 英文摘要: --
- 中文關鍵字: betel quid, carcinoma, DMBA, hamster, p53
- 英文關鍵字: --