- 作者: Wen-Bin Liu; Jian-Zhi Zhang; Bei-Hai Jiang; Ting-Ting Ren; Man-Man Gong; Lin Meng; Cheng-Chao Shou
- 作者服務機構: Peking University School of Oncology and Beijing Institute for Cancer Research, Beijing 100034, People's Republic of China
- 中文摘要: --
- 英文摘要: p37 protein is a membrane lipoprotein of Mycoplasma hyorhinis, and our previous work showed that there was high ratio of M. hyorhinis infection in human gastric carcinoma. To investigate the possible functions of p37 in cancer development, the nucleotide sequence of p37 gene was modified and expressed well in transfected cells. We found that p37 localized at the Golgi apparatus and could be secreted out of the cell. Human gastric cancer cells AGS, after being transfected with the p37 gene, were smaller, more spherical and easy to detach from each other. Their adhesion to matrix was also diminished and cytoskeleton in these stable p37 AGS cell was rearranged and transcription co-factor β-actin was transferred to nucleolus with down-regulation of ICAM-1 and integrin β1. These findings will be helpful for us to elucidate the effects of p37 on eukaryotic cells as well as to better understand the potential relationship between cancer and mycoplasma infection.
- 中文關鍵字: --
- 英文關鍵字: cell adhesion, Golgi apparatus, Mycoplasma hyorhinis, p37 protein