- 作者: Yun-Liang Yang; Hsing-Fang Chen; Tai-Jung Kuo; Chi-Yang Lin
- 作者服務機構: Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan, Republic of China
- 中文摘要: --
- 英文摘要: Enolase (2-phospho-D-glycerate hydrolase) is an enzymatic component of the glycolytic pathway and is conserved through evolution. The TR-CaENO1/Caenol stain, of which the expression of CaENO1 is under control of the tetracycline-regulatable (TR) expression system, is utilized for elucidating the functions of CaENOl in Candida albicans. As expected, there was no detectable CaENO1 mRNA when the TR-CaENO1/Caenol cells grew on media containing doxycycline repressing the expression of TR-Ca-ENO1.The TR-CaENO1/Caenol cells were arrested in media containing doxycycline in the presence of glucose but not in non-fermentable carbon sources, such as glycerol. Furthermore, the TR-CaENO1/ Caenol cells were also arrested in media containing 4% serum. In this study, we have showed that CaENO1 is required for the cell growth of C. albicans in the presence of glucose. Our findings may help us to design new and more effective antifungal agents for preventing and treating bloodstream fungal infections by blocking the function(s) of enolases.
- 中文關鍵字: --
- 英文關鍵字: Candida albicans, cell growth, enolase, glucose, inhibition