- 作者: Hao Yan, Haitao Yi, Lixin Xia, Zhenke Zhan, Weiyi He, Jijuan Cao, Ping-Chang Yang, Zhigang Liu
- 作者服務機構: The Center of allergy and clinical immunology, the First Affiliated Hospital of Liaoning Medical college, Jinzhou, China
- 中文摘要: --
- 英文摘要:
The epithelial barrier dysfunction plays a critical role in the pathogenesis of a broad array of immune diseases. Alix protein is involved in the endolysosome system. This study aims to elucidate the role of Alix in the maintenance of epithelial barrier function.
Results:The results showed that Alix was detected in T84 cells at both mRNA and protein levels. Exposure to Staphylococcal enterotoxin B (SEB) markedly suppressed the expression of Alix in T84 cells, which could be blocked by knocking down the Toll like receptor 2. The exposure to SEB did not affect the TER, but markedly increased the permeability of T84 monolayers to OVA; the OVA passing through T84 monolayers still preserved the antigenicity manifesting inducing antigen specific T cells proliferation.
Conclusions:Alix protein plays a critical role in the maintenance of the barrier function of T84 monolayers. - 中文關鍵字: --
- 英文關鍵字: Alix, Epithelial cell, Barrier function, Staphylococcal enterotoxin B, Antigenicity