- 作者: 林麗娟 ; 李寬容 ; 蕭明熙
- 作者服務機構: 榮民總醫院
- 中文摘要: The chromatographic behavior of eight lanostanoid triterpenes, which are four pairs of C-3 epimers and two pairs of C-3/C-15 positional isomers, were determined by reverse-phase high performance liquid chromatography (RP- HPLC). In the mobile phase systems of acetonitrile-water- acetic acid, methanol-water-acetic acid, and tetrahydrofuran-water-acetic acid, these triterpenes showed a very good linear relationship between capacity factors(k') and volume fractions of organic modifiers. The partition coefficients (Poct) of these triterpenes in 1- octanol/water and capacity factors (k') in RP-HPLC, when both expressed in logarithm, correlated linearly. This study showed that RP-HPLC is an effective method to evaluate the molecular hydrophobicity of multi-functional compounds which are stereo- and positional isomers.
- 英文摘要: --
- 中文關鍵字: Triterpene; Reversephase Hplc; Partition Coefficient; Capacity Factor
- 英文關鍵字: 三烯;逆相高效能液相層析法;分配係數;容積因子